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Evidence for the heparin-binding site of the A1 domain of fibronectin on epithelial cell surfaces.
We showed previously that the major heparin-binding sites of the A1-domain of fibronectin (FN) are located on the epithelial cell surface. Heparin-binding sites in various other cellular components (e.g., on nuclear surface, on cell surface, in collagen, and in proteoglycans) have been reported previously, but it remains unclear whether a mechanism exists by which such heparin-binding sites are activated on these surfaces. However, the possibility remains that the heparin-binding sites on the surface of epithelial cells are bound only to heparin and not to other polyanionic substrates. To rule out this possibility, we have examined the heparin-binding activity of the recombinant A1-domain of FN with commercially available reagents. Heparin-binding sites of the A1-domain of FN were specifically labeled with (125)I-heparin by incorporation of a sulfate group. Binding was inhibited by unlabeled heparin and by a low-molecular-weight heparin (pentosan polysulfate, CPS), but not by dextran sulfate, fucoidan, and heparitin. Binding of heparin to the A1-domain was observed with chick embryo fibroblasts, human foreskin fibroblasts, and human mammary carcinoma cells (MDA-MB-231). These findings demonstrate the presence of heparin-binding sites on the epithelial cell surface and also on a variety of other cell types. The identification of a putative heparin-binding site of fibronectin on the epithelial cell surface provides a possible explanation for the mechanism of activation of heparin-binding sites on this and other cell types.It’s official! Microsoft’s Windows Phone 7 will be available at major US wireless carriers at the end of the month. Here’s the plan, according to an iAdevs source:
Starting in the fourth quarter of this year, AT&T, T-Mobile and other major U.S. carriers will roll out Windows Phone 7 handsets. Starting in the first quarter of 2010, Verizon, which has a deal in place to sell Windows Mobile devices from HTC and Samsung, will start selling the phones in the United States.
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