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It has been well-documented that the application of electron crystallography (EC) to the determination of protein structure and dynamics has been an exceptionally valuable and relatively inexpensive tool. This is particularly true for domain-swapped proteins, in which rigid-body electron crystallography using macromolecular crystallography (MX) has produced an unprecedented structural characterisation of membrane proteins. By applying this technique, the structure of the ArcB and the HlyB proteins from E. coli have been established using nanoelectron crystallography (NEC) data. Surprisingly, these studies demonstrated that the two ArcB proteins in the same membrane are not in a dynamic equilibrium and that only one ArcB protein has the amino acid sequence of the closed conformation. In addition, these studies also demonstrated that the HlyB protein has an aqueous cavity between the two membrane leaflets. By applying NEC to the analysis of negatively stained protein crystals using cryo-electron microscopy (cryo-EM), the structure and dynamics of peripheral lipid-binding proteins have been established. This technology has revealed the well-known Adh-succinate dehydrogenase (SDH) dimer to have two hydrophobic ‘handles’ and the structural plasticity of the substrate-binding peripheral protein. The methodology and results of these diverse examples are discussed in this review, and the general principles of EC are applied to the derivation of accurate three-dimensional structures.Q:
How to calculate all combinations of size and weight of a box?
I’m trying to find all the combinations of size and weight of a box. Every combination should be different. The box doesn’t have to hold any elements.
Is there a formula to calculate this?
Ok, well, I have no idea why exactly you ask, but this is how you do it:
Calculate all the possible weights for the box.
For each weight, think of
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He had entered the Nehru Yuva Kendra, a non-governmental organization in the capital, on Saturday.
Kumar had to walk in the ‘sweet 16’ beauty pageant, along with other contestants from across India.
The challenge was designed to inspire